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ห้องสมุดกรมพัฒนาที่ดิน

3

Development model of vetiver-based bionematicide on controlling Root-knot
nematodes༛for safe chilli production

Abstract

The actual research outcome of this research project plan reveal that all sub-projects

can be completed within the specified timeframe and achieve the year one objectives.

According to the study's findings, the chemical composition of the Songkhla 3 vetiver grass
species from different planting sites was discovered to contain p-coumaric acid. The mortality

rate of root-knot nematodes was examined on these compounds at the laboratory level. It

was discovered that p-coumaric acid was able to kill 69.86 percent of root-knot nematodes
within 24 hours, compared to 36.78 percent of vetiver extracts. It was revealed that p-coumaric

acid belongs to the group of phenolic compounds. The greatest total phenolic compound

(TPC) content was found in a research of growing vetiver plants in a bioreactor system, and
the quantity of TPC extraction was higher when more BA was administered. Vetiver grass grown

in the TIB system at 30 – 45 days of age had the greatest TPC of 6.7 ug/mg and polysaccharide

content of 65.04 ug/mg when compared to natural vetiver extract, which had TPC
concentration in the range of 53.27 - 98.47 ug/mg and polysaccharide content in the range of

163.25 - 329.58 ug/mg. The efficacy of vetiver extract in controlling root-knot nematodes in

chili at both laboratory and greenhouse levels was found that vetiver extract had a killing
effect (>80 percent), inhibited root-knot nematode development (>70 percent), and

decreased egg number per root weight (>80 percent) without toxicity to the chilli in the

experiment. It also enhances the growth of chilli when used as green manure and as a
foundation soil mix. When the spray dry version of vetiver extract was evaluated in the lab, it

was discovered that a 50 mg/ml application rate was able to kill root-knot nematodes just as

effectively as the vetiver extracts. When the vetiver extract products were examined with chili
in the laboratories, the afore mentioned application rate reduced the number of eggs per root

weight by 90.14 percent, which was the same as the vetiver extract. As a consequence, vetiver

grass can be used to produce a solution to tackle root-knot nematodes. Farmers facing root
knot disease in chili fields will benefit from the expertise of vetiver grass extraction in the

future.

The second year of research will be based on extending the findings into field trials in
order to provide information on a more systematic approach to root-knot nematode