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ห้องสมุดกรมพัฒนาที่ดิน

จ

addition,༛V. zizanioides (L.)༛Nash༛extract༛from༛roots༛had༛higher༛TPC༛than༛extracts༛from༛

shoots༛and༛leaves.༛As༛for༛the༛multiplication༛stage༛of༛1༛month༛age,༛brown༛leaves༛that༛
occurred during cultured༛had༛the༛highest༛TPC༛and༛total༛polysaccharide༛content,༛which༛

were 6.70༛and 65.04༛µg/mg,༛respectively.༛In༛addition,༛total༛TPC༛was༛30.61༛µg/mg,༛which༛

(2
was higher than natural seedling from Khon Kaen source 5 . 0 2 ༛ µg/mg) and close to
Mahasarakham༛source (32.29 µg/mg) when extracted by boiling.

Bacterial contaminants in solid media systems for vetiver were identified as
Acinetobacter, Agrobacterium, Bacillus, Burkholderia, Kosakonia, Pantoea and Rhizobium,

whereas TIB systems were contaminated by Bacillus. Fusarium was the most fungal

contaminant in solid system and fungal contaminants in TIB were Cladosporium and
Fusarium solani.༛ These༛ results༛ indicated༛ that༛ endophytes༛ were༛ the༛ main༛ source༛ of༛

microbial༛contamination༛in༛solid༛system.༛Bacillus and Cladosporium contaminated in TIB
system༛suggested༛that༛caused༛by༛laboratory༛conditions,༛whereas༛Fusarium solani may

introduced༛by༛endophytic༛fungus༛of༛vetiver. It was found that 6%༛of༛hydrogen༛peroxide༛

could inhibit all tested bacteria.༛Bacillus tequilensis was༛resist༛to༛PPM༛(2ml/L)༛and༛0.1%༛of༛
streptomycin.༛Furthermore,༛0.1%༛of༛streptomycin༛could༛not༛inhibit༛species༛of༛Burkholderia

and Pantoea dispersa. Fungicidal༛ activity༛ of༛ carbendazim༛ (1-3%)༛ against༛ fungal༛

contaminants of vetiver micropropagation༛was༛carried༛out.༛The༛results༛found༛that༛1%༛of༛
carbendazim༛was༛sufficiently༛concentration༛for༛growth༛inhibition༛of༛fungal༛contaminants.

Keywords: Vetiveria zizanioides (L.)༛ Nash,༛ micropropagation༛ system,༛ plant༛ tissue༛

culture temporary immersion bioreactor,༛contamination,༛root-knot༛nematode,༛

product